Liver is significant to human life while liver disease is one of the greatest threats to human health. Since the occurrence and development of significant liver diseases is not only related with some individual genes or proteins and the research on liver proteome will construct the panoramic picture for liver physiological and pathologic processes, it's generally considered that uncovering the secret of significant liver diseases from the view of proteome is a novel and promising way. Consequently, Human Proteome Organization (HUPO) starts the Human Liver Proteome Project (HLPP) directed by China in 2002.
One major goal of HLPP is to study the binary protein-protein interactions between liver proteins and the important liver protein complexes. A protein array of about 5000 unique ORFs was screened by yeast two hybrid mating method. More than one thousand potential interactions were identified till now. And 1355 proteins were used as baits to screen human liver cDNA library by yeast two hybrid. Total 1889 unique protein-protein interactions were obtained, and 1270 interactions were verified by yeast retransformation assay. More than 55% of 54 random selected interactions were verified by co-immunoprecipitation assay. Our dataset has comparable confidence level to two large scale datasets published recently as is analyzed by web-based bioinformatics tool PICASSO. For our surprising, our data has little overlap (less than 1%) with two published large-scale human interactome dataset, which may indicate the low coverage rate of current human interactome. Furthermore, few protein complexes were identified by affinity method, which focus on HBV-related proteins and liver-specific enrichment transcription factors. More than 100 high confidence proteins were identified by mass spectrometry, which corresponding to HNF, AKT and HBx etc. The protein linkage map of human liver may be valuable source for human protein-protein interactome and liver diseases study.
Here we release protein-protein interaction dataset of HLPP. Moreover, to expand interaction network and provide valuable annotation and evidence for protein and interaction of HLPP dataset, we also collect and demonstrate protein-protein interaction which has been identified in experiment to happen between human and mammals from other renowned databases, including IntAct, HPRD, Bind, Mint and Dip. You may browse or search interaction and interactor flexibly, or you could view the interactive interaction network composed by interested interactor. Additionally, abundant gene annotation is available as well.